Composite
Part:BBa_K2814020:Design
Designed by: Saksham Sharma Group: iGEM18_IIT_Delhi (2018-10-10)
Third Node_ Recombinase based IFFL
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 946
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 27
Illegal BsaI site found at 966
Illegal SapI.rc site found at 130
Design Notes
Orientation of attP and attB sites was checked carefully
Source
We designed this part after going through the scientific literature.